Measuring very low affinity interactions between immunoglobulin superfamily cell-adhesion molecules
van der Merwe PA, Brown MH, Davis SJ, Barclay AN. (1993), Biochem Soc Trans. 21, 340S
Stored sCD48-CD4 (0.6 mg/ml, aggregates) and size-fractionated sCD48-CD4 (0.6 mg/ml, no aggregates) were injected in HBS for 15 s over dextran matrix with nothing (Control) or with rat sCD2 (CD2) immobilized. HBS contains (mM): NaCl 150, CaCl2 1, MgCl2 1, Na Azide 10, Hepes 25 (pH 7.5), and Sufactant P20 0.005% (w/vol). All experiments performed at 37°C at flow-rate of 20 μl/min.